Method for Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas9
Walter, Schubert, Kung, Hawkins, Platt, Hernday, Mahatdejkul-Meadows, Szeto, Chandran, Newman, Horwitz (2019) Method for Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas9 Methods Mol Biol (IF: -1) 2049 39-72Abstract
CRISPR-Cas has proven to be a powerful tool for precision genetic engineering in a variety of difficult genetic systems. In the highly tractable yeast S. cerevisiae, CRISPR-Cas can be used to conduct multiple engineering steps in parallel, allowing for engineering of complex metabolic pathways at multiple genomic loci in as little as 1 week. In addition, CRISPR-Cas can be used to consolidate multiple causal alleles into a single strain, bypassing the laborious traditional methods using marked constructs, or mating. These tools compress the engineering timeline sixfold or more, greatly increasing the productivity of the strain engineer.
Links
http://www.ncbi.nlm.nih.gov/pubmed/31602604http://dx.doi.org/10.1007/978-1-4939-9736-7_3
