Optimized Production of Lentiviral Vectors for CAR-T Cell
Moço, de Abreu Neto, Fantacini, Picanço-Castro (2020) Optimized Production of Lentiviral Vectors for CAR-T Cell Methods Mol Biol (IF: -1) 2086 69-76Abstract
Advances in the use of lentiviral vectors for gene therapy applications have created a need for large-scale manufacture of clinical-grade viral vectors for transfer of genetic materials. Lentiviral vectors can transduce a wide range of cell types and integrate into the host genome of dividing and nondividing cells, resulting in long-term expression of the transgene both in vitro and in vivo. In this chapter, we present a method to transfect human cells, creating an easy platform to produce lentiviral vectors for CAR-T cell application.
Links
http://www.ncbi.nlm.nih.gov/pubmed/31707668http://dx.doi.org/10.1007/978-1-0716-0146-4_5
