Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors
Mo, Mamonkin (2020) Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors Methods Mol Biol (IF: -1) 2086 119-130Abstract
Manufacturing chimeric antigen receptor (CAR)-modified T cells requires incorporation of the CAR transgene, for which viral vectors are most often used. Here, we describe the generation of CAR T cells using primary human T cells and a non-self-inactivating gammaretroviral vector encoding a CAR transgene. The gammaretroviral vector is produced by 293T cells transiently transfected with DNA plasmids encoding necessary components of the viral vector. The resulting viral particles efficiently infect activated T cells and integrate the CAR transgene into the genome of dividing cells for stable expression.
Links
http://www.ncbi.nlm.nih.gov/pubmed/31707671http://dx.doi.org/10.1007/978-1-0716-0146-4_8
