Coupled liquid chromatography-gas chromatography for the rapid analysis of gamma-oryzanol in rice lipids
Miller, Frenzel, Schmarr, Engel (2003) Coupled liquid chromatography-gas chromatography for the rapid analysis of gamma-oryzanol in rice lipids J Chromatogr A (IF: 4) 985(1-2) 403-10Abstract
An approach based on on-line coupled liquid chromatography-gas chromatography (LC-GC) was developed for the rapid analysis of gamma-oryzanol in rice. Total lipids were extracted from rice and subjected to LC-GC without any prior purification. gamma-Oryzanol was pre-separated by HPLC from rice lipids and transferred on-line to GC analysis in order to separate its major constituents. 24-methylenecycloartanyl ferulate, cycloartenyl ferulate, campesteryl ferulate, beta-sitosteryl ferulate and campestanyl ferulate. The identities of the compounds were confirmed by off-line GC-MS analysis. Total gamma-oryzanol content could be quantified by HPLC-UV detection and the distribution of gamma-oryzanol constituents could be determined by on-line coupled GC analysis. The proposed methodology paves the way for high-throughput investigations providing information on natural variations in gamma-oryzanol content and its composition in different rice varieties.
Links
http://www.ncbi.nlm.nih.gov/pubmed/12580509http://dx.doi.org/10.1016/s0021-9673(02)01238-4
