Evaluation of three diagnostic methods, including real-time PCR, for detection of Dientamoeba fragilis in stool specimens

Stark, Beebe, Marriott, Ellis, Harkness (2006) Evaluation of three diagnostic methods, including real-time PCR, for detection of Dientamoeba fragilis in stool specimens J Clin Microbiol (IF: 5.4) 44(1) 232-5

Abstract

Dientamoeba fragilis is a protozoan parasite of humans that infects the mucosa of the large intestine and is associated with gastrointestinal disease. We developed a 5' nuclease (TaqMan)-based real-time PCR assay, targeting the small subunit rRNA gene, for the detection of D. fragilis in human stool specimens and compared its sensitivity and specificity to conventional PCR and microscopic examination by a traditional modified iron-hematoxylin staining procedure. Real-time PCR exhibited 100% sensitivity and specificity.

Links

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1351980
http://www.ncbi.nlm.nih.gov/pubmed/16390978
http://dx.doi.org/10.1128/JCM.44.1.232-235.2006

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