Single-channel currents underlying glycinergic inhibitory postsynaptic responses in spinal neurons
Takahashi, Momiyama (1991) Single-channel currents underlying glycinergic inhibitory postsynaptic responses in spinal neurons Neuron (IF: 14.7) 7(6) 965-9Abstract
Single-channel properties of glycine receptors have been characterized so far only in cultured neurons. To characterize the glycine receptor channels in situ, we applied the patch-clamp technique to spinal neurons in slice preparations. Glycine-gated, single-channel currents were recorded in outside-out patches excised from spinal neurons. In the falling phase of glycinergic inhibitory synaptic currents, single-channel currents were resolved as discrete steps. In both cases, the glycine-gated channels showed similar multiple conductance levels. These results suggest that the receptor channel properties are indistinguishable in the synaptic and extrasynaptic sites. We conclude that multiple conductance states of a receptor channel are the native feature of the glycine receptor in situ.
Links
http://www.ncbi.nlm.nih.gov/pubmed/1722412http://dx.doi.org/10.1016/0896-6273(91)90341-v
