The flexizyme system: a highly flexible tRNA aminoacylation tool for the translation apparatus

Masaki Ohuchi, Hiroshi Murakami, Hiroaki Suga (2007) The flexizyme system: a highly flexible tRNA aminoacylation tool for the translation apparatus Curr Opin Chem Biol (IF: 6.1) 11(5) 537-42

Abstract

Flexizymes are de novo ribozymes capable of charging a wide variety of non-natural amino acids on tRNAs. The flexizyme system enables reprogramming of the genetic code by reassigning the codons that are generally assigned to natural amino acids to non-natural residues, and thus mRNA-directed synthesis of non-natural polypeptides can be achieved. In this review, we comprehensively summarize the history of the flexizyme system and its subsequent development into a practical tool. Furthermore, applications to the synthesis of novel biopolymers via genetic code reprogramming and perspectives for future applications are described.

Links

http://www.ncbi.nlm.nih.gov/pubmed/17884697
http://dx.doi.org/10.1016/j.cbpa.2007.08.011

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