Membrane isolation on polylysine-coated beads. Plasma membrane from HeLa cells

Cohen, Kalish, Jacobson, Branton (1977) Membrane isolation on polylysine-coated beads. Plasma membrane from HeLa cells J Cell Biol (IF: 7.4) 75(1) 119-34

Abstract

HeLa cell plasma membranes have been purified after binding cells to polylysine-coated polyacrylamide beads. Cell attachment to beads and membrane recovery were maximal in a sucrose-acetate buffer, pH 5.0, at 25 degrees C. Measurements of ouabain-sensitive NaK-adenosine triphosphatase, membrane-bound 125I-wheat germ agglutinin, and chemical analyses showed that membranes on beads were of comparable or greater purity than membranes isolated by conventional methods. Because the isolation procedure is rapid (approximately 2.5 h), and produces membranes whose protoplasmic surfaces are fully exposed, it should be a useful supplement to standard isolation techniques.

Links

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111560
http://www.ncbi.nlm.nih.gov/pubmed/21192
http://dx.doi.org/10.1083/jcb.75.1.119

Similar articles

Tools

Export to RIS