Dynamic light scattering: a practical guide and applications in biomedical sciences

Jörg Stetefeld, Sean McKenna, Trushar Patel (2016) Dynamic light scattering: a practical guide and applications in biomedical sciences Biophys Rev (IF: 3.7) 8(4) 409-427

Abstract

Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. The diffusion coefficient, and hence the hydrodynamic radii calculated from it, depends on the size and shape of macromolecules. In this review, we provide evidence of the usefulness of DLS to study the homogeneity of proteins, nucleic acids, and complexes of protein-protein or protein-nucleic acid preparations, as well as to study protein-small molecule interactions. Further, we provide examples of DLS's application both as a complementary method to analytical ultracentrifugation studies and as a screening tool to validate solution scattering models using determined hydrodynamic radii.

Links

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425802
http://www.ncbi.nlm.nih.gov/pubmed/28510011
http://dx.doi.org/10.1007/s12551-016-0218-6

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