Ginsenoside Re enhances the survival of H9c2 cardiac muscle cells through regulation of autophagy
Zhang, Liu, Huang, Liang, Zhang, Fan, Ma (2020) Ginsenoside Re enhances the survival of H9c2 cardiac muscle cells through regulation of autophagy J Asian Nat Prod Res (IF: 1.7) 22(8) 774-787Abstract
We examined the effect of ginsenoside Re (G-Re) on autophagy in H9c2 cardiomyocytes cultured in glucose deprivation (GD). Levels of the membrane-bound autophagy-related microtubule-associated protein 1A/1B-light chain 3 (LC3) B-2 were measured via immunoblotting and immunofluorescence was conducted to assess autophagosome formation. GD H9c2 cells were treated with 100 μmol/l G-Re. Cell viability was determined in culture medium. Phosphorylated 5' AMP-activated protein kinase (AMPK)-α and mammalian target of rapamycin (mTOR) levels were measured to explore the mechanisms underlying the effects of G-Re on autophagy in GD cells. G-Re treatment inhibited autophagosome formation and may be beneficial to GD cardiomyocytes.
Links
http://www.ncbi.nlm.nih.gov/pubmed/31232107http://dx.doi.org/10.1080/10286020.2019.1632834
