A one-step tRNA-CRISPR system for genome-wide genetic interaction mapping in mammalian cells

Zhao, Tyrishkin, Sjaarda, Khanal, Stafford, Rauh, Liu, Babak, Yang (2019) A one-step tRNA-CRISPR system for genome-wide genetic interaction mapping in mammalian cells Sci Rep (IF: 4.6) 9(1) 14499

Abstract

Mapping genetic interactions in mammalian cells is limited due to technical obstacles. Here we describe a method called TCGI (tRNA-CRISPR for genetic interactions) to generate a high-efficient, barcode-free and scalable pairwise CRISPR libraries in mammalian cells for identifying genetic interactions. We have generated a genome- wide library to identify genes genetically interacting with TAZ in cell viability regulation. Validation of candidate synergistic genes reveals the screening accuracy of 85% and TAZ-MCL1 is characterized as combinational drug targets for non-small cell lung cancer treatments. TCGI has dramatically improved the current methods for mapping genetic interactions and screening drug targets for combinational therapies.

Links

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787096
http://www.ncbi.nlm.nih.gov/pubmed/31601883
http://dx.doi.org/10.1038/s41598-019-51090-3

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