Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays
Nacasaki Silvestre, Moço, Picanço-Castro (2020) Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays Methods Mol Biol (IF: -1) 2086 213-222Abstract
Immunotherapy using T cells modified with chimeric antigen receptor (CAR) has been proven effective in the treatment of leukemia and lymphomas resistant to chemotherapy. Recent clinical studies have shown excellent responses of CAR-T cells in a variety of B cell tumors. However, it is important to validate in vitro activity of these cells, though different sorts of assays, which are capable of measuring the cytotoxic potential of these cells. In this chapter, it will be pointed two methods to evaluate CAR-T cell killing potential against B cell malignancy cell lines.
Links
http://www.ncbi.nlm.nih.gov/pubmed/31707679http://dx.doi.org/10.1007/978-1-0716-0146-4_16
