Ji, Hou, Gao, Xue, Yan, Guo (2020) Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.) polysaccharides in a colorectal cancer mouse model Food & function 11(1) 163-173


Accumulating evidence has reported that the gut microbiota could play important roles in the occurrence and progression of colorectal cancer. The nondigestible plant polysaccharides have always been fermented by the intestinal microbiota. Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models. However, the molecular mechanisms involved in polysaccharides preventing carcinogenesis are still uncharacterized. The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing. Contrasted with control group, the addition of JP could ward off colon cancer by ameliorating colitis cancer-induced gut dysbiosis. In addition, there was a significant decrease in Firmicutes/Bacteroidetes post JP treatment. What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks. These results suggested that JP showed prebiotic-like activities by positively modulating intestinal microbiota and affecting certain metabolic pathways contributing to host health. In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.

越来越多的证据表明,肠道菌群可能在结直肠癌的发生和发展中起重要作用。不可消化的植物多糖一直被肠道菌群发酵。枣果实中发现的主要功能成分多糖已被证明可抑制动物模型的致癌作用。然而,涉及多糖预防癌变的分子机制仍然未知。这项研究的目的是研究大枣多糖(JP)对肠道菌群的调节作用,然后使用AOM / DSS诱导的结肠炎小鼠模型,通过高通量分析JP对肠道菌群结构的影响。排序。与对照组相比,添加JP可以减轻结肠炎引起的肠道营养不良,从而预防结肠癌。此外,JP治疗后的坚定/拟杆菌显着减少。此外,JP干预13周后,代谢途径的KEGG途径,ATP结合盒(ABC)转运蛋白和两组分系统丰富了差异最大的表达基因。这些结果表明,JP通过积极调节肠道菌群并影响有助于宿主健康的某些代谢途径而显示出类似益生元的活性。总之,我们的结果证明了JP具有恢复AOM / DSS改变的肠道菌群特性的显着能力,表明枣多糖作为预防和治疗结肠直肠癌的有希望的益生元候选物的潜力。



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