A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression
Chen, Sun, Li, Zhang (2020) A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression Chem Commun (Camb) (IF: -1) 56(4) 627-630Abstract
Here, we report a novel dual-functional microRNA (miRNA) probe, PA-miRNA, for miRNA target identification and light control of miRNA target expression. PA-miRNA is a miRNA mimic with a 3'-biotin tag linked via a photo-cleavable linker. Using PA-miR-34a, intracellular targets of miR-34a in HeLa cells were isolated and confirmed. Moreover, PA-miR-34a upon transfection into HeLa cells was inactive until light irradiation to break the photo-cleavable linker to release functional miR-34a. We demonstrated that miR-34a target expression as well as miR-34a-promoted cell apoptosis were regulated by PA-miR-34a in a photo-controllable manner.
Links
http://www.ncbi.nlm.nih.gov/pubmed/31833483http://dx.doi.org/10.1039/c9cc08277h
