Detection of Ascorbate Peroxidase (APX) Activity in Plant Tissues: Using Non-denaturing PAGE and Spectrophotometric Assay
Rodríguez-Ruiz, Houmani, Muñoz-Vargas, Palma, Corpas (2024) Detection of Ascorbate Peroxidase (APX) Activity in Plant Tissues: Using Non-denaturing PAGE and Spectrophotometric Assay Methods Mol Biol (IF: -1) 2798 223-234Abstract
At the cellular level, the generation of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2), due to different abiotic or biotic stress, causes oxidative stress that induces an imbalance in the metabolism. Among the different H2O2-scavenging enzymatic antioxidants, ascorbate peroxidase (APX) is a heme-peroxidase that plays an important role in the ascorbate-glutathione pathway using ascorbate to reduce H2O2 to water. Using non-denaturing polyacrylamide gel electrophoresis (PAGE) in combination with a spectrophotometric assay for APX activity, the protocol allows identifying diverse APX isozymes present in different organs and plant species.© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Links
http://www.ncbi.nlm.nih.gov/pubmed/38587747http://dx.doi.org/10.1007/978-1-0716-3826-2_16
