Mutation detection by denaturing gradient gel electrophoresis (DGGE)
Fodde, Losekoot (1994) Mutation detection by denaturing gradient gel electrophoresis (DGGE) Hum Mutat (IF: 3.3) 3(2) 83-94Abstract
The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically amplified DNA. The technique is based on the migration of double-stranded DNA molecules through polyacrylamide gels containing linearly increasing concentrations of a denaturing agent. In this review DGGE and the several modifications of the original protocol are presented. Moreover, its applications in human molecular genetics are summarized together with a preliminary comparison with other mutation detection technologies such as chemical cleavage, RNase protection, and single-strand conformation polymorphism.
Links
http://www.ncbi.nlm.nih.gov/pubmed/8199599http://dx.doi.org/10.1002/humu.1380030202
